Table 1.
Primer Sequences for Real-Time PCR
| Gene name | Forward, 5’>3’ | Reverse, 5’>3’ |
|---|---|---|
| Actb | GGCTGTATTCCCCTCCATCG | CCAGTTGGTAACAATGCCATGT |
| Pepck1 | CGCTGGATGTCGGAAGAGG | GGCGAGTCTGTCAGTTCAATAC |
| G6pc | CGACTCGCTATCTCCAAGTGA | GTTGAACCAGTCTCCGACCA |
| Pgc1a | TATGGAGTGACATAGAGTGTGCT | CCACTTCAATCCACCCAGAAAG |
| Mkp-3 | TGCGGGCGAGTTCAAATACA | AGCAATGCACCAGGACACCA |
| Obr/OBRa | GGATATTGGAGTAATTGGAGCA | GATTTCCCACATCTTCTGACCA |
| Obra | GTTGTTTTGGGACGATGTTCCA | TGGGTTCATCTGTAGTGGTCATG |
| Obrb | GACGATGTTCCAAACCCCAAGA | GCTCCAGAAGAAGAGGACCAAA |
| Obrc | GTTGTTTTGGGACGATGTTCCA | TGGCATCTAAACTGCAACCTTAG |
| ACTB | TCACCATGGATGATGATATCGC | ATAGGAATCCTTCTGACCCATGC |
| PEPCK1 | TTGAGAAAGCGTTCAATGCCA | CACGTAGGGTGAATCCGTCAG |
| G6PC | GTGTCCGTGATCGCAGACC | GACGAGGTTGAGCCAGTCTC |
| PGC1A | TGAAGACGGATTGCCCTCATT | GCTGGTGCCAGTAAGAGCTT |
a
Primers for Obr and OBR shared the same sequences; gene names with capital letters refer to human genes.