Figure 5.

Vaccination of F508del mice with CVB vaccines prevent virus-mediated organ damage

F508del mice were buffer-treated (n = 10) or vaccinated with CVB1 or CVB3 vaccines (n = 13) and infected with CVB1 or CVB3 virus, as shown in Figure 3A. Organs were collected on day 4 post-infection for histological analysis of organ integrity by haematoxylin and eosin (H&E) staining of formalin fixed paraffin embedded sections.

(A) Pancreas scores of buffer + CVB or vaccine + CVB groups. Pancreas were scored according to exocrine damage and the presence of infiltrating immune cells.

(B) Parenchymal health, (C) parenchymal damage and (D) hemosiderin laden particle staining in the lungs of buffer-treated + CVB-infected or vaccinated + CVB-infected mice. For (A)–(D), a score of 0 indicates healthy tissue and a score of 4 indicates highly damaged tissue. Each mouse is represented by an individual symbol and shown are the mean values ±SD ∗p < 0.05, ∗∗∗∗p < 0.001, as compared by the unpaired t-test.

(E−L) Representative images of H&E-stained pancreas (E−H) and lung (I–L) from buffer + CVB1 (E and I), CVB1 vaccine + CVB1 (F and J), buffer + CVB3 (G and K), and CVB3 vaccine + CVB (H and L)-treated F508del mice on day 4 post-infection. In (I), the insert is a 5× magnification of the original image and the yellow arrows show hemosiderin laden particles. See also Figures S5 and S6.