Antibodies |
|
FITC anti-mouse CD4 |
Biolegend |
Clone RM4-5; Cat#100510; RRID:AB_312713
|
PE anti-mouse TCR beta chain |
Biolegend |
Clone H57-597; Cat#109207; RRID:AB_313430
|
APC anti-mouse/human CD45R/B220 |
Biolegend |
Clone RA3-6B2; Cat# 103212; RRID:AB_312997
|
APC anti-mouse CD8a |
Biolegend |
Clone 53-6.7; Cat#100712; RRID:AB_312751
|
|
Bacterial and virus strains |
|
CVB1 (neutralizing antibody assay, Finland) |
VacTech Oy, Finland |
V200WE3C |
CVB2 (neutralizing antibody assay, Finland) |
VacTech Oy, Finland |
V166XE4c |
CVB3 (neutralizing antibody assay, Finland) |
VacTech Oy, Finland |
V13D4c |
CVB4 (neutralizing antibody assay, Finland) |
VacTech Oy, Finland |
VD33SD4c |
CVB5 (neutralizing antibody assay, Finland) |
VacTech Oy, Finland |
V10D3c |
CVB6 (neutralizing antibody assay, Finland) |
VacTech Oy, Finland |
V147UD3c |
Polio 1, Sabin (neutralizing antibody assay, Finland) |
Terveyden ja hyvinvoinnin laitos – THL, Finland |
MOPV1 |
Polio 3, Sabin (neutralizing antibody assay, Finland) |
Terveyden ja hyvinvoinnin laitos – THL, Finland |
MOPV3 |
CVB3-Nancy (neutralizing antibody assay, Sweden and vaccine, Finland) |
Dr Gun Frisk, Uppsala University, Uppsala, Sweden |
|
CVB1-10796 wild-type strain (Argentina; vaccine and mouse infection studies) |
CDC, USA |
|
CVB3 wild-type strain (Finland; vaccine) |
Hus lab, Helsinki, Finland |
|
|
Biological samples |
|
Human serum (CF patients) |
Stockholm CF Center, Karolinska University Hospital, Huddinge |
n/a |
Human serum (healthy controls) |
Unrelated vaccine studies performed at Center for Infectious Medicine, KI |
n/a |
|
Chemicals, peptides, and recombinant proteins |
|
Coxsackievirus B1 antiserum |
ATCC |
V-028-501-560 |
Coxsackievirus B2 antiserum |
ATCC |
V-029-501-560 |
Coxsackievirus B3 antiserum |
ATCC |
V-030-501-560 |
Coxsackievirus B4 antiserum |
ATCC |
V-031-501-560 |
Coxsackievirus B5 antiserum |
ATCC |
V-032-501-560 |
Coxsackievirus B6 antiserum |
ATCC |
V-033-501-563 |
|
Experimental models: Cell lines |
|
Green monkey kidney (GMK) cells |
National Institute for Health and Welfare, Finland |
RRID:CVCL_L878 |
HeLa cells |
Dr. R Glas, Center for Infectious Medicine. |
RRID:CVCL_0030 |
Vero cells |
ATCC |
RRID:CVCL_0059 |
|
Experimental models: Organisms/strains |
|
B6.129S2-Tcratm1Mom/J |
Jackson Laboratories |
RRID:IMSR_JAX:002115 |
C57Bl/6J strain 000664 |
Jackson Laboratories |
RRID:IMSR_JAX:000664 |
Cftrtm1EUR
|
Dr Bob Scholte; Erasmus University, Rotterdam, The Netherlands |
MGI:1857899
|
|
Oligonucleotides |
|
Primer TCRα KO mice genotyping mutant reverse: CTA CCC GCT TCC ATT GCT C |
Invitrogen Custom Primers |
Jackson Laboratories |
Primer TCRα KO mice genotyping common: TGA CTC CCA AAT CAA TGT GC |
Invitrogen Custom Primers |
Jackson Laboratories |
Primer TCRα KO mice genotyping wild-type reverse: GGT GAG ATG ACC CAA AGC AG |
Invitrogen Custom Primers |
Jackson Laboratories |
Primer F508del mice genotyping CF-P581: CAC AAC ACT GAC ACA AGT AGC |
Invitrogen Custom Primers |
van Doornick et al., 1995 |
Primer F508del mice genotyping CF-P580: GGA CGC AAA GAA AGG GAT AAG |
Invitrogen Custom Primers |
van Doornick et al., 1995 |
|
Software and algorithms |
|
GraphPad 8.3.1 |
Graphpad |
www.graphpad.com |
FlowJo |
FlowJo |
www.flowjo.com |
BioRender |
BioRender |
www.biorender.com |