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. 2022 Sep 5;25(10):105070. doi: 10.1016/j.isci.2022.105070
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

FITC anti-mouse CD4 Biolegend Clone RM4-5; Cat#100510; RRID:AB_312713
PE anti-mouse TCR beta chain Biolegend Clone H57-597; Cat#109207; RRID:AB_313430
APC anti-mouse/human CD45R/B220 Biolegend Clone RA3-6B2; Cat# 103212; RRID:AB_312997
APC anti-mouse CD8a Biolegend Clone 53-6.7; Cat#100712; RRID:AB_312751

Bacterial and virus strains

CVB1 (neutralizing antibody assay, Finland) VacTech Oy, Finland V200WE3C
CVB2 (neutralizing antibody assay, Finland) VacTech Oy, Finland V166XE4c
CVB3 (neutralizing antibody assay, Finland) VacTech Oy, Finland V13D4c
CVB4 (neutralizing antibody assay, Finland) VacTech Oy, Finland VD33SD4c
CVB5 (neutralizing antibody assay, Finland) VacTech Oy, Finland V10D3c
CVB6 (neutralizing antibody assay, Finland) VacTech Oy, Finland V147UD3c
Polio 1, Sabin (neutralizing antibody assay, Finland) Terveyden ja hyvinvoinnin laitos – THL, Finland MOPV1
Polio 3, Sabin (neutralizing antibody assay, Finland) Terveyden ja hyvinvoinnin laitos – THL, Finland MOPV3
CVB3-Nancy (neutralizing antibody assay, Sweden and vaccine, Finland) Dr Gun Frisk, Uppsala University, Uppsala, Sweden
CVB1-10796 wild-type strain (Argentina; vaccine and mouse infection studies) CDC, USA
CVB3 wild-type strain (Finland; vaccine) Hus lab, Helsinki, Finland

Biological samples

Human serum (CF patients) Stockholm CF Center, Karolinska University Hospital, Huddinge n/a
Human serum (healthy controls) Unrelated vaccine studies performed at Center for Infectious Medicine, KI n/a

Chemicals, peptides, and recombinant proteins

Coxsackievirus B1 antiserum ATCC V-028-501-560
Coxsackievirus B2 antiserum ATCC V-029-501-560
Coxsackievirus B3 antiserum ATCC V-030-501-560
Coxsackievirus B4 antiserum ATCC V-031-501-560
Coxsackievirus B5 antiserum ATCC V-032-501-560
Coxsackievirus B6 antiserum ATCC V-033-501-563

Experimental models: Cell lines

Green monkey kidney (GMK) cells National Institute for Health and Welfare, Finland RRID:CVCL_L878
HeLa cells Dr. R Glas, Center for Infectious Medicine. RRID:CVCL_0030
Vero cells ATCC RRID:CVCL_0059

Experimental models: Organisms/strains

B6.129S2-Tcratm1Mom/J Jackson Laboratories RRID:IMSR_JAX:002115
C57Bl/6J strain 000664 Jackson Laboratories RRID:IMSR_JAX:000664
Cftrtm1EUR Dr Bob Scholte; Erasmus University, Rotterdam, The Netherlands MGI:1857899

Oligonucleotides

Primer TCRα KO mice genotyping mutant reverse: CTA CCC GCT TCC ATT GCT C Invitrogen Custom Primers Jackson Laboratories
Primer TCRα KO mice genotyping common: TGA CTC CCA AAT CAA TGT GC Invitrogen Custom Primers Jackson Laboratories
Primer TCRα KO mice genotyping wild-type reverse: GGT GAG ATG ACC CAA AGC AG Invitrogen Custom Primers Jackson Laboratories
Primer F508del mice genotyping CF-P581: CAC AAC ACT GAC ACA AGT AGC Invitrogen Custom Primers van Doornick et al., 1995
Primer F508del mice genotyping CF-P580: GGA CGC AAA GAA AGG GAT AAG Invitrogen Custom Primers van Doornick et al., 1995

Software and algorithms

GraphPad 8.3.1 Graphpad www.graphpad.com
FlowJo FlowJo www.flowjo.com
BioRender BioRender www.biorender.com