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. 2022 Aug 19;298(10):102405. doi: 10.1016/j.jbc.2022.102405

Figure 8.

Figure 8

Downregulation of Cdc20 induces apoptosis in H460 cells.A, gene expression analysis of Cdc20 in normal lung and in the lung of adenocarcinoma patients using The Cancer Genome Atlas database. B, H460 cells were transfected with either control (Ctl) or Cdc20 siRNA. After 48 h, cells were collected and cell lysates were subjected to immunoblotting analysis with antibody probes specific for Cdc20 and securin. Immunoblotting with anti-β-actin IgGs was performed as control. Ponceau S staining shows equal total protein loading. Quantification of protein band intensity is shown at the bottom of each blot. C–E, H460 cells were transfected with either control (Ctl) or Cdc20 siRNA. After 48 h, cells were subjected to crystal violet staining (C) (Scale bar = 2 mm), DAPI staining (D) (Scale bar = 50 μm), and immunoblotting analysis with anti-caspase-3 IgGs (E). F, H460 cells were transfected with increasing concentrations of Cdc20 siRNA (2.5, 5, 10, 20, and 40 pmol). As control, H460 cells were transfected with 40 pmol of control (Ctl) siRNA. After 48 h, cell survival was quantified by MTT assay. Quantification is shown. Values in (F) represent means ± SEM; statistical comparisons were made using the student’s t test. ∗p < 0.001.