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. 2022 Sep 7;13:971251. doi: 10.3389/fphys.2022.971251

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Copyright © 2022 Rosenbaek, Petrillo, van Bemmelen, Staub, Murali and Fenton.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Nedd4-2 interacts with NCC. (A) Three E3 ubiquitin ligases were found in a Y2H assay with the C-terminus of NCC as bait. Nedd4-2, Nedd4, and Pdzrn3. The orange line represents the Selected Interaction Domain (SID), corresponding to the amino acid sequence shared by all prey fragments matching the same reference protein. (B) NCC immunoprecipitation (IP) of Nedd4-2 from MDCKI-hNCC cells. IP was performed using FLAG-M2 affinity beads and negative controls were performed in the absence of antibody (A,B).