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. 2022 Sep 7;13:971251. doi: 10.3389/fphys.2022.971251

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Copyright © 2022 Rosenbaek, Petrillo, van Bemmelen, Staub, Murali and Fenton.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Nedd4-2 mRNA and protein levels in MDCKI-hNCC Nedd4-2 knockdown (N4-2 KD) cell lines. (A) mRNA levels of Nedd4-2 (left) and NCC (right) in MDCKI-hNCC Ctrl and N4-2 KD cell lines. The relative mRNA abundances are calculated from the Ct values using the delta-delta Ct method. Data are means ± S.E.M. (n = 9) *p < 0.05 assessed by unpaired Student’s t test. (B) Representative immunoblots of Nedd4-2, NCC, and proteasome 20S in MDCKI-hNCC Ctrl and N4-2 KD cell lines.