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. 2022 Sep 7;13:971251. doi: 10.3389/fphys.2022.971251

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Copyright © 2022 Rosenbaek, Petrillo, van Bemmelen, Staub, Murali and Fenton.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Nedd4-2 deficiency increases total NCC levels and plasma membrane abundance. Semi-quantitative assessment of Nedd4-2 (A), NCC (B), and pT58-NCC (C) levels in total cell lysates of MDCKI-hNCC Ctrl and Nedd4-2 (N4-2) KD cell lines. Data are means ± S.E.M. (n = 9) *p < 0.05 assessed by unpaired Student’s t-test. (D) Representative blots of Nedd4-2, NCC, and pT58-NCC in total cell lysates of MDCKI-hNCC Ctrl and N4-2 KD cell lines. Semi-quantitative assessment of NCC (E), pT58-NCC (F) and the NCC/pT58-NCC ratio (G) in membrane fractions of MDCKI-hNCC Ctrl and Nedd4-2 (N4-2) KD cell lines. Data are means ± S.E.M. (n = 9) *p < 0.05 assessed by unpaired Student’s t-test. (H) Representative blots of NCC and pT58-NCC in membrane fractions of MDCKI-hNCC Ctrl and N4-2 KD cell lines.