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. 2022 Sep 7;13:971251. doi: 10.3389/fphys.2022.971251

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Copyright © 2022 Rosenbaek, Petrillo, van Bemmelen, Staub, Murali and Fenton.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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No significant difference in SPAK and phosphorylated SPAK (pS373-SPAK) are found in MDCKI-hNCC cells with Nedd4-2 KD. Semi-quantitative assessment of SPAK (A), pS373-SPAK (B), and pS373-SPAK/SPAK ratio (C) in total cell lysates of MDCKI-hNCC Ctrl and Nedd4-2 (N4-2) KD cell lines. Data are means ± S.E.M. (n = 9) *p < 0.05 assessed by unpaired Student’s t-test. (D) Representative blots of SPAK and pS373-SPAK in total cell lysates of MDCKI-hNCC Ctrl and N4-2 KD cell lines.