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. 2022 Sep 7;13:971251. doi: 10.3389/fphys.2022.971251

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Copyright © 2022 Rosenbaek, Petrillo, van Bemmelen, Staub, Murali and Fenton.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ubiquitylation levels of NCC in total lysates and membrane fraction are decreased after Nedd4-2 knock down. Semi-quantitative assessment of ubiquitylation levels of NCC in total cell lysates (A) and membrane fractions (B) of MDCKI-hNCC Ctrl and Nedd4-2 (N4-2) KD cell lines. Data are means ± S.E.M. (n = 9) *p < 0.05 assessed by unpaired Student’s t-test. Representative immunoblots of ubiquitylated NCC and total NCC from total cell lysates are shown in (C) and the membrane fraction in (D).