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. 2022 Sep 7;13:971251. doi: 10.3389/fphys.2022.971251

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Copyright © 2022 Rosenbaek, Petrillo, van Bemmelen, Staub, Murali and Fenton.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NCC half-life is increased with Nedd4-2 deficiency in MDCKI-hNCC cells. (A) Semi-quantitative assessment of NCC protein half-life from 8 independent experiments. Data are means ± S.E.M. *p < 0.05 assessed by unpaired Student’s t-test. (B) Representative immunoblots of NCC in cell lysates isolated from MDCKI-hNCC Ctrl and Nedd4-2 (N4-2) KD cells treated with cycloheximide and actinomycin for the indicated timepoints. Half-lives were calculated for each experiment with a one-phase exponential decay analysis.