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. 2022 Sep 7;13:971251. doi: 10.3389/fphys.2022.971251

FIGURE 8.

FIGURE 8

Nedd4-2 is not involved in the potassium induced regulation of NCC and pT58-NCC. Semi-quantitative assessment of Nedd4-2 (A), NCC, pT58-NCC, and pT58-NCC/NCC (B+C) levels in tubules isolated from control and Nedd4-2 KO mice and treated with 0.5 mM, 3.5 mM, or 8.0 mM K+ for 24 h. (A+B) Data are means ± S.E.M and normalized to control tubules treated with 3.5 mM K+. Difference between control and KO tubules treated with either 0.5 mM, 3.5 mM, or 8.0 mM K+ is assessed by 2way ANOVA followed by Tukey’s multiple comparisons test (*p < 0.05). (C) Data are means ± S.E.M. Control and KO tubules are normalized to their own 3.5 mM K+ group. Differences of NCC and pT58-NCC levels with different K+ concentrations in either control or KO tubules are assessed by 2way ANOVA followed by Tukey’s multiple comparisons test (*p < 0.05). Fold changes between control and KO tubules with changing K+ concentrations are assessed in the same 2way ANOVA analysis (n = 16–20). (D) Representative immunoblots of Nedd4-2, NCC, pT58-NCC, and proteasome 20S in control and KO tubules treated with 0.5 mM, 3.5 mM, or 8.0 mM K+.