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. 2022 Aug 1;14(5):1077–1101. doi: 10.1016/j.jcmgh.2022.07.014

Figure 5.

Figure 5

Neutrophil depletion showed no significant protective effect on FVH progression. (A) Anti-LY6G was injected intraperitoneally at 24 and 4 hours before, and 12 hours after MHV-3 infection to deplete neutrophils as the time shaft indicated. Rat IGg2a was used as a control. (B) The depletion efficacy of neutrophils at 48 and 72 hours after infection was verified by immunochemical staining of LY6G. Arrows indicate representative areas of neutrophils. Scale bars: 50 μm. (C) The overall survival rate of mice was observed (n = 15–18 in each group). Survival rate was compared by using the log-rank test. (D) Hepatic NETs formation after neutrophil depletion was detected by immunofluorescent staining (Cit-H3, red; NE, green). (E) Liver enzymes (ALT, AST) and (F) liver histology was examined at 72 hours after MHV-3 infection. For bar graphs, n = 5 in each group. Statistical analysis was performed by unpaired Student t test. ∗∗∗P < .001, ∗∗∗∗P < .0001. DAPI, 4′,6-diamidino-2-phenylindole.