Figure 8.

FGL2 promoted accumulation of hepatic neutrophils via enhanced expression of ICAM-1, Cxcl1/2, and Cxcr2 in MHV-3 infection. Liver neutrophil infiltration was detected by Ly6G staining using (A) immunohistochemistry and (B) flow cytometry gated by CD45+CD11b+LY6G+. Scale bars: 50 μm. (C) Protein levels of hepatic ICAM-1, E-selectin, and L-selectin were measured by Western blot and analyzed by Image lab. (D) Relative mRNA levels of Cxcl1, Cxcl2, Cxcl5, and Cxcr2 in liver were measured by quantitative reverse-transcription polymerase chain reaction and expressed as a ratio to β-actin. For bar graphs, n = 5 in each group. ∗P < .05, ∗∗P < .01, ∗∗∗P < .001, and ∗∗∗∗P < .0001, determined by 1-way analysis of variance followed by the Tukey follow-up test. CXCL, Chemokine (C-X-C Motif) Ligand; CXCR, CXC chemokine receptor.