Fig. 4.

PIM acylation is rapid. A–D: HPTLC of PIMs during a 40-min treatment with (A) 0 mM (Rf = 0.18–0.45), (B) 25 mM (Rf = 0.18–0.45), (C) 50 mM (Rf = 0.21–0.47), and (D) 100 mM benzyl alcohol (Rf = 0.21–0.47). HPTLC was performed and PIMs were visualized as in Fig. 2B. Representative image of triplicate experiments is shown. For quantification, HPTLC plates were scanned, and band intensities of PIM species were quantified as mannose content (see Materials and methods for details). Average ± standard deviation is shown in the graphs. BA, benzyl alcohol; HPTLC, high-performance thin layer chromatography; PIM, phosphatidylinositol mannoside.