Fig. 5.

Inhibition of protein synthesis does not affect PIM inositol acylation. A: Growth of M. smegmatis treated with 10 μg/ml chloramphenicol measured by OD₆₀₀ over 24 h. Experiments were performed in triplicate, and average ± standard deviation is shown. B: Cells were treated with 10 μg/ml chloramphenicol or methanol (vehicle control) for 10 min followed by 100 mM benzyl alcohol or DMSO (vehicle control) for 60 min. HPTLC analysis of PIMs is as described in Fig. 2B (Rf = 0.14–0.42). Experiment was repeated twice, and a representative result is shown. BA, benzyl alcohol; CHL, chloramphenicol; DMSO, dimethyl sulfoxide; HPTLC, high-performance thin layer chromatography; PIM, phosphatidylinositol mannoside.