Fig. 7.

Inositol acylation in response to known membrane fluidizers. HPTLC analysis of PIMs extracted from M. smegmatis cells treated with (A) aromatic alcohols (Rf = 0.16–0.38), (B) straight-chain alcohols (Rf = 0.17–0.39), or (C) other chemicals (Rf = 0.17–0.45). Cells were treated for 1 h at 37°C with 100 mM alcohols (except hexanol and octanol at 50 mM and 4 mM, respectively), 600 μg/ml dibucaine, 1 mM carvacrol, 50 ng/ml bedaquiline, 25 μM CCCP, and 10 μg/ml chloramphenicol. Chloramphenicol was included as a control, which does not target the membrane (also see Fig. 5). HPTLC was performed as in Fig. 2B. All experiments were repeated at least twice, and representative results are shown. CCCP, carbonyl cyanide m-chlorophenyl hydrazone; HPTLC, high-performance thin layer chromatography; PIM, phosphatidylinositol mannoside.