Fig. 9.

Heat shock induces PIM acylation. A: HPTLC analysis of PIMs after heating cells at 42°C, 50°C, and 55°C (Rf = 0.18–0.45, 0.18–0.45, and 0.15–0.41, respectively). B: Cells during the 55°C heat shock were serially diluted and plated onto Middlebrook 7H10 agar, confirming that cells remained viable at this temperature. C: HPTLC analysis of PIMs from cells that were heat shocked at 55°C for 1 h and recovered at 37°C for 5 h (Rf = 0.18–0.43). Data shown for panels A–C are representative results from two independent experiments. D: Recovery after heat shock at 42°C (blue circle) or 55°C (red square). The black arrow indicates when heat shock was stopped (at 1 h). The experiment was done in triplicate. HPTLC, high-performance thin layer chromatography; PIM, phosphatidylinositol mannoside.