REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
Fluo-4 Direct Calcium Assay Kit	Thermo Fisher Scientific	ref #F10471 https://www.thermofisher.com/order/catalog/product/F10471?SID=srch-hj-F10471
Deionized water	N/A	N/A
MEM (Minimal Essential Medium)	Thermo Fisher Scientific	ref #31095052 https://www.thermofisher.com/order/catalog/product/31095052
MEM, suspension, no calcium, no glutamine (S-MEM)	Thermo Fisher Scientific	ref #11380037 https://www.thermofisher.com/order/catalog/product/11380037?SID=srch-hj-11380037
Calcium Chloride solution (CaCl2); 1 M	Honeywell	ref #21114; CAS: 10043-52-4 https://lab.honeywell.com/shop/calcium-chloride-solution-21114
Trypsin - EDTA 10× - 100 mL	Dutscher	ref #X0930-100 https://www.dutscher.com/frontoffice/article/X0930-100
Dulbecco’s Phosphate Buffered Saline (PBS without Ca2+, Mg2+)	Merck	ref #D8537 https://www.sigmaaldrich.com/FR/fr/product/sigma/d8537?gclid=CjwKCAjwrqqSBhBbEiwAlQeqGvLyhOJR9S-2gniYubfDfVLmk5zl6V-fIUDZ3sm-B0FYvp5RR7jkERoCsxIQAvD_BwE
Penicillin/streptomycin 10 000 U/10 mg/mL	Dutscher	ref #P06-07100 https://www.dutscher.com/frontoffice/article/P06-07100#
RIPA Lysis Buffer, 10×	Merck	ref #20-188; CAS: 127087-87-0, 83-44-3 https://www.sigmaaldrich.com/FR/fr/product/mm/20188
Sylgard 184 (Elastomer + curing agent)	Neyco	ref #DC184-1.1 https://www.neyco.fr/en/our-products/silicones-1/microfluidic-pdms/sylgard-184-silicone/silicones-2/microfluidique-pdms/silicone-sylgard-184/sylgard-184
3-aminopropyltriethoxysilane (APTES)	Merck	ref #440140; CAS: 919-30-2 https://www.sigmaaldrich.com/FR/en/product/aldrich/440140
FluoSpheres™ Carboxylate-Modified Microspheres (2.0 μm, blue fluorescent 365/415 nm)	Thermo Fisher Scientific	ref #F8824 https://www.thermofisher.com/order/catalog/product/F8824
Deposited data
Microsystem designs	N/A	Mendeley Data, V1, https://doi.org/10.17632/vdhwrpxw2s.1
Experimental models: Cell lines
MDCK–II Cell line canine	Merck	ref #00062107 https://www.sigmaaldrich.com/FR/en/product/sigma/00062107
Software and algorithms
ImageJ	National Institutes of Health	https://imagej.nih.gov/ij/download.html
Microsoft Excel	Microsoft	https://www.microsoft.com/en-gb/microsoft-365/excel
AutoCAD Software	Autodesk	https://www.autodesk.com/products/autocad/overview?term=1-YEAR&tab=subscription
Other
Polycarbonate - Film (Thickness: 0.375 mm)	Goodfellow	ref #CT30-FM-000140 https://www.goodfellow.com/fr/en-us/displayitemdetails/p/ct30-fm-000140/polycarbonate-film
Elastosil Film 2030 250/20 (Thickness: 20 μm)	WACKER	https://www.wacker.com/h/en-us/c/elastosil-film-2030/p/000038005
Low pressure plasma system (Plasma Cleaner) Zepto Model 3 13.56 MHz base unit type B incl. rotary switch control	Diener	https://www.plasma.com/en/low-pressureplasmasystem-zepto/
Microfluidic pump AF1 VAN & AF1 VAC	ELVEFLOW	https://www.elveflow.com/microfluidic-products/microfluidics-flow-control-systems/high-accuracy-vacuum-pressure-pumps-af1/
Biopsy punch 1 mm	World Precision Instruments	ref #504646 https://www.wpiinc.com/var-949-reusable-biopsy-punch-various-sizes
PTFE pipe ø int. x ø ext. = 0.5 × 1 mm length 25 m	Dutscher	ref #091927 https://www.dutscher.com/frontoffice/article/091927
Ultrasound tank, 1.4 L	Dutscher	ref #053275 https://www.dutscher.com/frontoffice/article/053275