TABLE 1.
B. bronchiseptica strains and plasmids used in this study
| Strain or plasmid | Relevant genotype, phenotype, or description | Reference or source |
|---|---|---|
| Strains | ||
| B013N | Nalidixic acid-resistant derivative of strain B013, derived from swine isolate B | 2 |
| BRM1 | B013N alcA::mini-Tn5 lacZ1; Kanr; alcaligin siderophore deficient | 2 |
| BRM6 | B013N alcC::mini-Tn5 lacZ1; Kanr; alcaligin siderophore deficient | 2 |
| BRM9 | B013N alcA::mini-Tn5 lacZ1, with mini-Tn5 fusion element in antisense orientation with respect to alcA transcription; Kanr; alcaligin siderophore deficient | 2 |
| BRM13 | BRM1 ΔalcR1 | This study |
| BRM14 | BRM6 ΔalcR1 | This study |
| BRM15 | BRM9 ΔalcR1 | This study |
| Plasmids | ||
| pRK415 | Mobilizable broad-host-range plasmid cloning vector; Tetr; RK2 origin | 27 |
| pRK21 | pRK415 carrying 1.6-kb KpnI-PstI DNA insert fragment of B. pertussis UT25; alcR+; Tetr; formerly designated pP9KP | 6 |
| pRK15 | pRK415 carrying 2.3-kb EcoRI-PstI DNA insert fragment of B. bronchiseptica B013N; alcR+; Tetr | This study |
| pRK16 | pRK15, except 2.1-kb ΔalcR1 EcoRI-PstI DNA insert fragment of B. bronchiseptica B013N | This study |