FIGURE 7.

Differentially expressed categories of genes [(A) = 4-fold downregulated, (B) = 4-fold upregulated] relative to the control (−N + P). Results are from transcript-based Gene Ontology (GO) enrichment analysis. The significant differential expression of genes was determined using Sleuth (Wald test; qval < 0.05), and gene categories were tested for significant differential expression using GOenrich (hypergeometric distribution analysis; padj < 0.05). Padj values were Log₁₀-transformed for scaling purposes, and green - red colorations corresponded to lower - higher degrees of significance. Gene categories plotted were those associated with nitrogen (N) and phosphorus (P) assimilation, growth (G), as well as photosynthesis (Photo), while superscripts correspond to biological ^(B), cellular ^(C), and molecular ^(M) gene categories. The number of differentially expressed genes (DEGs) in gene categories are represented by circle size.