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. 2022 Feb 2;71(5):1081–1098. doi: 10.2337/db21-0536

Figure 3.

Figure 3

Phenotypic characterization of T2DM-hCSCs in vitro. A: Flow cytometry dot plots showing ROS in T2DM-hCSCs compared with NDM-hCSCs (representative of n = 3 experiments). B: Bar graphs showing telomerase activity and average telomere length in T2DM-hCSCs compared with NDM-hCSCs (n = 6 biological replicates). Data are mean ± SD. C: Representative Western blot showing p16INK4a, p21, and p53 levels in T2DM-hCSCs compared with NDM-hCSCs (n = 3 biological replicates). D: Bar graph and representative confocal microscopy images from cytospin preparations showing p16INK4a expression (red) in T2DM-hCSCs compared with NDM-hCSCs. Scale bar = 75 μm (n = 6 biological replicates). E: Representative confocal microscopy image from cytospin preparation of T2DM-hCSCs coexpressing the senescent markers p16INK4a (green) and p21 (red). Scale bar = 75 μm. (n = 6 biological replicates). F: Bar graph and representative light microscopy images showing senescence-associated β-gal–positive cells (blue) in T2DM-hCSCs compared with NDM-hCSCs. Scale bar = 200 μm. (n = 6 biological replicates). G and H: Bar graphs and representative confocal microscopy images from cytospin preparations of c T2DM-hCSCs and NDM-hCSCs showing the expression of γ-H2AX (green) and terminal deoxynucleotidyl transferase (TdT) (green) in T2DM-hCSCs compared with NDM-hCSCs. Scale bars = 75 μm and 50 μm (n = 6 biological replicates). Data are mean ± SD. NEG. CTRL, negative control.