FIG. 7.

PFGE of the S. suis genomic DNA digested with SmaI. Electrophoresis was carried out with a CHEF system for 20 h at 200 V and 10°C with pulse time ramping from 5 to 50 s in a 1% agarose gel. Strains carrying the R-M system were as follows: DAT1 (lane 1), 205 (lane 2), 220 (lane 3), 227 (lane 4), 243 (lane 5), 222 (lane 6), and 236 (lane 7). Strains with the null phenotype were as follows: NCTC10234 (lane 8), 204 (lane 9), 207 (lane 10), 209 (lane 11), 210 (lane 12), 213 (lane 13), and 246 (lane 14). S, λ DNA concatemers as size standards.