TABLE 3.
Predicted gene products and the homology exhibited by their potential protein products to amino acid sequences in the databasea
Predicted gene | Coding position
|
Product size
|
Predicted pI | Homology
|
Description and function of closest relativeb | |||
---|---|---|---|---|---|---|---|---|
Start | Stop | aac | kDa | BLAST score | aa identityd | |||
purF | <1 | 940 | >312 | >34.6 | 4.67 | e-124 | 73/300 | Phosphoribosylpyrophosphate amidotransferase precursor of L. lactis (EC 2.4.2.14) [U64311] |
purM | 995 | 2017 | 340 | 36.4 | 4.54 | e-128 | 69/339 | Phosphoribosylformylglycineamide cycloligase of L. lactis subsp. cremoris (EC 6.3.3.1) [AF016634] |
purN | 2014 | 2565 | 183 | 20.5 | 4.88 | 8e-58 | 71/150 | Phosphoribosylglycineamide formyltransferase homolog of S. pyogenes (EC 22.1.2.2) [U70775] |
purH | 2575 | 4122 | 515 | 56.6 | 4.83 | e-169 | 58/513 | Phosphoribosylamidoimidazole carboxyamide formyltransferase of B. subtilis (EC 6.3.2.6) [J02732] |
ssuMA | 4187 | 5017 | 276 | 31.8 | 4.93 | e-114 | 71/275 | M.LlaDCHI A of L. lactis [U16027] |
e-110 | 70/274 | M.DpnII 1 of S. pneumoniae [M14339] | ||||||
ssuMB | 5007 | 5822 | 271 | 31.2 | 9.50 | e-145 | 90/266 | M.DpnA of S. pneumoniae [M14339] |
e-139 | 86/263 | M.LlaDCHI B of L. lactis [U16027] | ||||||
ssuRA | 5800 | 6726 | 308 | 36.0 | 5.20 | 3e-78 | 49/299 | R.DpnII of S. pneumoniae [M14339] |
ssuRB | 6726 | 7631 | 301 | 34.4 | 5.17 | e-126 | 72/300 | R.LlaDCHI of L. lactis [U16027] |
purD | 7752 | 9014 | 420 | 45.3 | 4.48 | e-125 | 54/419 | Phosphoribosylamineglycine ligase of L. lactis (EC 6.3.4.13) [AJ000883] |
purE | 9040 | 9528 | 162 | 16.8 | 6.36 | 4e-41 | 61/139 | Phosphoribosylamidoimidazole carboxylase catalytic subunit of B. subtilis (EC 4.1.1.21) [J02732] |
Proteins relating to purine biosynthesis have been named according to their closest relative, probable function, and E. coli gene designations (60). Coding region positions are numbered with respect to the entire 9,618-bp sequence determined (AB045609) and from the first base of the start codon to the last base of the stop codon. The Genetyx-Mac program (Software Developing Company) was used to make predictions of coding regions, molecular masses, and isoelectric points.
Numbers in blackets are accession numbers in the DDBJ/EMBL/GenBank database.
aa, amino acids.
Percent identity/number of amino acids evaluated.