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. 2022 Aug 18;298(10):102401. doi: 10.1016/j.jbc.2022.102401

Figure 3.

Figure 3

Hepatic depletion of ACLY alone or both ACLY and ACSS2 decrease hepatic AcCoA levels but increase DNL and liver TG in mice fed HFD. Liver homogenates were processed to measure specific metabolite changes resulting from depletion of ACLY and/or ACSS2. A, hepatic AcCoA levels. B, plasma acetate levels. C, hepatic MalCoA levels measured by LC–MS/MS. D, hepatic newly synthesized palmitate levels (measured by deuterium incorporation from D2O into liver palmitate). E, hepatic total palmitate levels. F, liver TG levels per milligram of protein. (ns, not significant, ∗p < 0.05, ∗∗p < 0.005, ∗∗∗p < 0.0005, and ∗∗∗∗p < 0.00005). AcCoA, acetyl CoA; ACLY, ATP-citrate lyase; ACSS2, AcCoA synthase short-chain family member 2; DNL, de novo lipogenesis; HFD, high-fat diet; MalCoA, malonyl CoA; TG, triglyceride.