FIG. 2.

Expression of gafD peptides in rifampin-treated pulse-labeled E. coli cells and in the periplasm of G fimbria-expressing E. coli strains. (A) Autoradiographic analysis of ¹⁴C-labeled peptides of E. coli BL21 λDE3(pKJ1) immediately after addition of ¹⁴C-amino acids (lane 1) and 1 h 30 min later (lane 2). (B) Western blotting with anti-GafD antibodies of periplasmic peptides prepared from recombinant E. coli strains BL21 λDE3(pRR-5), KS474(pRR-5), IHE11088(pRR-5), BL21 λDE3(pKJ1) (lanes 1 to 4, respectively) and from wild-type E. coli strain IHE11165 (lane 5). Plasmid pRR-5 contains the entire gaf gene cluster, and pKJ1 encodes GafD1-321. The migration distance of the ΔGafD peptide is indicated on the right.