Adult male B6D2F1 mice (4/group) were fed ad libitum for 1 week on an isocaloric semi-purified diet of the indicated protein calorie percentage. (A) Body weight change expressed as percentage of starting weight.
(B) Cumulative food intake normalized to body weight.
(C–E) Body weight percentage change (C), fat mass (D), and lean mass (E) after 1 week on the indicated diet.
(F–H) Four-hour-fasted values for (F) blood glucose, (G) serum insulin, and (H) FGF21.
Error bars are standard deviations, different letters indicate p < 0.05 in Tukey post-hoc test following significant one-way ANOVA F test. See also Figure S1 and Table S1.