FIG. 4.

Expression of katE and cbpA in the crr::kan mutant. (A) Strains CH12 (wild type [WT], katE-lacZ) and NM5 (crr::kan katE-lacZ), each harboring plasmids as described for Fig. 3A, were grown at 37°C in Luria broth supplemented with chloramphenicol (25 μg/ml). At the mid-logarithmic phase, β-galactosidase activity expressed by the katE-lacZ operon fusion was measured. The data are means with standard deviations for four independent assays. (B) A set of transformants as described for Fig. 3A was grown at 37°C in Luria broth supplemented with 25 μg of chloramphenicol per ml. Immunoblotting with anti-CbpA antiserum was carried out as described for Fig. 3A.