FIGURE 3.

Enzymatic property of EhLOx wild‐type and Met207Leu mutant. (a) Oxidase activity of wild‐type (red) and Met207Leu mutant (blue). (b) Dye‐mediated dehydrogenase activity of wild‐type (red) and Met207Leu mutant (blue). (c) Substrate specificity of wild‐type and Met207Leu mutant evaluated by dehydrogenase activity using 20 mM each substrate. (d) Thermal stability of wild‐type and Met207Leu mutant evaluated by dehydrogenase activity assay after 10 min incubation at each temperature. All assays were conducted using 20 mM PPB pH 7.0 at 25°C