TABLE 1.
The oxidase and dehydrogenase activities of EhLOx mutants
| Met207 substitutions | Oxidase activity (Ox) | Dehydrogenase activity (Dh) | Dh/Ox (%) | Relative expression Level (%)* | ||
|---|---|---|---|---|---|---|
| U/mg | % (vs. wild‐type) | U/mg | % (vs. wild‐type) | |||
| Gly | 1.2 ± 0.0 | 8.9% | 4.6 ± 0.0 | 20% | 400% | 83 |
| Ala | 3.0 ± 0.0 | 26% | 7.1 ± 0.1 | 36% | 240% | 73 |
| Val | 1.3 ± 0.0 | 9.1% | 6.2 ± 0.2 | 26% | 490% | 88 |
| Leu | 0.24 ± 0.00 | 1.6% | 25 ± 1 | 96% | 10,000% | 95 |
| Ile | 0.68 ± 0.02 | 4.3% | 20 ± 1 | 83% | 3,300% | 86 |
| Met (wild‐type) | 16 ± 0 | 100% | 27 ± 2 | 100% | 170% | 100 |
| Pro | 1.2 ± 0.0 | 9.2% | 2.0 ± 0.4 | 8.9% | 170% | 83 |
| Phe | 0.0024 ± 0.0001 | 0.037% | 0.038 ± 0.003 | 0.34% | 1,600% | 41 |
| Trp | 0.032 ± 0.001 | 0.23% | 0.093 ± 0.004 | 0.37% | 290% | 91 |
| Ser | 2.8 ± 0.1 | 19% | 6.0 ± 0.2 | 23% | 210% | 94 |
| Thr | 0.99 ± 0.01 | 7.3% | 5.2 ± 0.0 | 22% | 520% | 87 |
| Cys | 1.7 ± 0.1 | 11% | 8.1 ± 0.20 | 31% | 480% | 95 |
| Asn | 0.30 ± 0.01 | 1.7% | 2.2 ± 0.1 | 7.1% | 740% | 120 |
| Gln | 1.5 ± 0.0 | 11% | 5.2 ± 0.1 | 20% | 340% | 93 |
| Tyr | 0.93 ± 0.02 | 6.5% | 5.2 ± 0.1 | 21% | 560% | 91 |
| Asp | 0.048 ± 0.002 | 0.32% | 0.15 ± 0.00 | 0.58% | 320% | 96 |
| Glu | 0.13 ± 0.00 | 0.94% | 0.34 ± 0.02 | 1.4% | 260% | 86 |
| Lys | 0.018 ± 0.000 | 0.11% | 0.22 ± 0.00 | 0.82% | 1,200% | 98 |
| Arg | 0.021 ± 0.000 | 0.13% | 0.33 ± 0.00 | 1.2% | 1,600% | 100 |
| His | 0.12 ± 0.00 | 0.83% | 5.8 ± 0.2 | 23% | 4,800% | 93 |
Note: These expression levels were calculated from SDS‐PAGE band intensity (*Figure S2).