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. 2022 Sep 21;19:62. doi: 10.1186/s12989-022-00502-w

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Evaluation of DNA damages induced by GO sheets after repeated exposure. A Quantification of lung DNA damages using rabbit anti-mouse γ-H2Ax-Phosphorilated Ser139 immunostaining after repeated exposure to 3 × 10 µg (high dose) of GO sheets (USGO or LGO) and MWCNTs (positive control/reference materials), expressed as fold change in fluorescence intensity (negative control = water for injection). B Percentage of γ-H2Ax imunoreactivity (fluorescence intensity) located in lung immune cell infiltrates or in lung parenchyma at day 1, 7, 28 and 84 after repeated exposure to 3 × 10 µg. C Quantification of lung DNA damages after repeated exposure to 3 × 1 µg (low dose) of materials. D Percentage of γ-H2Ax imunoreactivity (fluorescence intensity) located in lung immune cell infiltrates or in lung parenchyma at day 1, 7 and 28 after repeated exposure to 3 × 1 µg. E Total number of γ-H2Ax positive cells in lung parenchyma (outside inflammatory infiltrates) expressed as fold change in fluorescence intensity, after repeated exposure to 3 × 10 µg. F Percentage of E-cadherin⁺ γ-H2Ax⁺ cells and CD45⁺ γ-H2Ax⁺ cells in lung parenchyma at day 1, 7, 28 and 84 after exposure. G Total number of γ-H2Ax positive cells in lung parenchyma (outside inflammatory infiltrates) expressed as fold change in fluorescence intensity, after repeated exposure to 3 × 1 µg. H Percentage of E-cadherin⁺ γ-H2Ax⁺ cells and CD45⁺ γ-H2Ax⁺ cells in lung parenchyma at day 1, 7 and 28 after exposure. Significance level *p < 0.05 **p < 0.01, ***p < 0.001 (One-Way ANOVA; n = 3)