Fig. 5.

Single-cell unsupervised clustering and annotation. Uniform manifold approximation and projection (UMAP) representation of the pooled cells depicted in different colours by A cluster and cell type and B sample. C Canonical cell markers were used to annotate clusters. Each colour represents the expression level (grey means low and purple means high). D Bar plot of cell fractions in patients with COPD and healthy controls in each cluster. E Cell fractions of all clusters in patients with COPD and healthy controls