TABLE 3.
Expression of operons encoding ribosomal proteins in an exponential-phase culture in LB medium
| Operon (min) | Genea | Proteinb | ppm (100) | Rank |
|---|---|---|---|---|
| Spc (73) | rplN | L14 | 8.6 | 122 |
| rplX | L24 | 27.1 | 31 | |
| (TR) | ||||
| rplE | L5 | 10.0 | 102 | |
| rpsN | S14 | 17.2 | 59 | |
| rpsH | S8 | 13.7 | 79 | |
| rplF | L6 | 6.9 | 167 | |
| rplR | L18 | 14.9 | 70 | |
| rpsE | S5 | 11.6 | 87 | |
| rpmD | L30 | 5.4 | 227 | |
| rplO | L15 | 5.8 | 206 | |
| secY | SecY | 1.9 | 1053 | |
| rpmJ | L36 | 4.0 | 359 | |
| S10 (73) | (TR) | |||
| rpsJ | S10 | 24.6 | 35 | |
| rplC | L3 | 23.9 | 41 | |
| rplD | L4 | 24.0 | 40 | |
| rplW | L23 | 43.9 | 7 | |
| rplB | L2 | 24.6 | 36 | |
| rpsS | S19 | 22.8 | 45 | |
| rplV | L22 | 15.7 | 66 | |
| rpsC | S3 | 27.4 | 29 | |
| rplP | L16 | 19.1 | 53 | |
| rpmC | L29 | 13.6 | 80 | |
| rpsQ | S17 | 7.8 | 142 | |
| α (73) | (TR) | |||
| rpsM | S13 | 8.1 | 136 | |
| rpsK | S11 | 8.4 | 128 | |
| rpsD | S4 | 15.3 | 67 | |
| rpoA | α | 32.6 | 23 | |
| rplQ | L17 | 7.1 | 163 | |
| Str (73) | rpsLc | S12 | 0.9 | 2291 |
| (TR) | ||||
| rpsG | S7 | 10.0 | 99 | |
| fusA | EF-G | 38.5 | 12 | |
| tufA | EF-Tu | 24.1 | 31 | |
| L11 (90) | (TR) | |||
| rplK | L11 | 68.0 | 2 | |
| rplA | L1 | 48.0 | 4 | |
| L10 or β (90) | (TR) | |||
| rplJ | L10 | 43.4 | 8 | |
| rplL | L7/12 | 66.4 | 3 | |
| (TT) | ||||
| rpoB | β | 11.4 | 89 | |
| rpoC | β′ | 5.0 | 258 | |
| S15 (69) | (TR) | |||
| rpsO | S15 | 16.0 | 63 | |
| (TT) | ||||
| pnp | Pnp | 5.7 | 215 | |
| S2 (4) | rpsB | S2 | 16.9 | 60 |
| tsf | EF-Ts | 14.2 | 76 | |
| L34 (83) | rpmH | L34 | 6.9 | 166 |
| mpA | RNAse P | 5.3 | 237 | |
| L28 (82) | rpmB | L28 | 33.5 | 20 |
| rpmG | L33 | 27.5 | 28 | |
| L35 (38) | infC | IF3 | 70.2 | 1 |
| (TR) | ||||
| rpmI | L35 | 46.2 | 6 | |
| rplT | L20 | 27.2 | 30 | |
| S20 (0) | rpsT | S20 | 5.3 | 235 |
| S1 (21) | rpsA | S1 | 23.8 | 43 |
| S6 (95) | rpsF | S6 | 38.3 | 14 |
| priB | n | 26.4 | 32 | |
| rpsR | S18 | 19.0 | 54 | |
| rplI | L9 | 30.6 | 25 | |
| S21 or ς (67) | rpsU | S21 | 17.3 | 58 |
| dnaG | DnaG | 2.1 | 875 | |
| rpoD | ς70 | 4.2 | 876 | |
| L25 (48) | rplY | L25 | 16.7 | 61 |
| L28 (82) | rpmB | L28 | 33.5 | 20 |
| rpmG | L33 | 27.5 | 28 | |
| L32 (24) | yceDd | ORF | 1.4 | 1463 |
| rpmF | L32 | 9.7 | 107 | |
| L13 (70) | rplM | L13 | 28.0 | 27 |
| rpsl | S9 | 8.0 | 140 | |
| trmD (57) | rpsP | S16 | 11.2 | 90 |
| b2608 | ORF | 10.1 | 98 | |
| trmD | TrmD | 32.3 | 24 | |
| rplS | L19 | 6.3 | 185 |
a
Genes within operons are listed in the order in which they are transcribed. TR, sites of translational repression; TT, sites of transcriptional termination (15).
b
Ribosomal proteins are indicated by their standard alphanumeric designations (15). Other proteins are involved in translation (EF-G, EF-Tu, EF-Ts, and IF3), secretion (SecY), transcription (α, β, β′, and ς70), RNA maturation and turnover (RNase P, TrmD, and Pnp), or replication (n and DnaG) (32).
c
Multiple PCR products were derived from the rpsL-specific amplification.
d
The yceD product was not of the expected size.