TABLE 4.
Expression of some amino acid biosynthetic operons and the PTS operon in an exponential-phase culture in minimal medium
| Operon | Genea | ppm (100) | Rank |
|---|---|---|---|
| Histidine | hisLb | 3.3 | 556 |
| hisG | 5.6 | 265 | |
| hisD | 2.5 | 818 | |
| hisC | 3.7 | 490 | |
| hisB | 3.3 | 555 | |
| hisH | 2.1 | 1,000 | |
| hisA | 1.8 | 1,185 | |
| hisF | 2.5 | 824 | |
| hisI | 4.2 | 417 | |
| Isoleucine-valine | ilvG | 7.5 | 171 |
| ilvM | 2.5 | 827 | |
| ilvE | 2.9 | 668 | |
| ilvD | 1.6 | 1,360 | |
| ilvA | 0.6 | 3,038 | |
| IRc regulator | ilvY∗∗ | 0.4 | 3,373 |
| IR | ilvC∗ | 41.7 | 5 |
| ALSd I | ilvB | 1.3 | 1,765 |
| ilvN | 1.7 | 1,295 | |
| ALS III | ilvIb | 2.9 | 662 |
| ilvH | 3.0 | 632 | |
| Leucine | leuA | 11.3 | 82 |
| leuB | 10.9 | 93 | |
| leuC | 6.3 | 215 | |
| leuD | 9.6 | 115 | |
| Threonine | thrL | 16.8 | 41 |
| thrA | 4.0 | 442 | |
| thrB | 5.4 | 276 | |
| thrC | 7.9 | 160 | |
| Tryptophan | trpL | 6.6 | 199 |
| trpE | 6.8 | 189 | |
| trpD | 4.3 | 396 | |
| trpC | 10.4 | 102 | |
| trpB | 11.6 | 77 | |
| trpA | 13.2 | 64 | |
| Proline AB | proA | 2.5 | 835 |
| proB | 2.4 | 863 | |
| Proline C | proC | 1.1 | 1,994 |
| Arginine E | argE∗∗ | 1.6 | 1,353 |
| Arginine CBH | argC∗ | 2.0 | 1,088 |
| argB∗ | 2.9 | 684 | |
| argH∗ | 2.2 | 967 | |
| PTS | ptsH | 31.0 | 12 |
| ptsI | 19.6 | 30 | |
| crr | 11.9 | 74 |
a
Genes within operons are listed in the order in which they are transcribed (2), except for the divergent clusters, where the sites of initiation lie between the genes labeled with one and two asterisks.
b
Multiple PCR products were derived from the ilvI- and hisL-specific amplifications.
c
IR, isomeroreductase.
d
ALS, acetolactate synthase.