TABLE 5.
Performance of molecular methods (compared with the gold standard reference method) currently in clinical use for H. pylori detection, according to recent reportsa
| Test | Gold standard method | Sensitivity (%) | Specificity (%) | PPV (%) | NPV (%) | Accuracy (%) | Reference |
|---|---|---|---|---|---|---|---|
| Nested PCR | UBT | 93.8 | 95.9 | 299 | |||
| Multiplex PCR | CIM and CLO | 100 | 59.6 | 73.8 | 100 | 81.1 | 300 |
| BACTfish H. pylori Combi test (Izinta Kft., Budapest, Hungary) | Histology | 98 | 99.6 | 98.9 | 99.2 | 250 | |
| RIDA GENE H. pylori (R-Biopharm AG, Darmstadt, Germany) | Culture alone or histopathology and RUT combined | 100 | 99 | 94 | 100 | 318 | |
| Amplidiag H. pylori+ClariR (Mobidiag, Espoo, Finland) | Culture or TaqMan RT-PCR | 96.3 | 98.7 | 92.2 | 99.3 | 319 | |
| MutaREAL H pylori (Inmundiagnostik, Bensheim, Germany) | Culture | 93.3 | 86.9 | 90.3 | 90.9 | 320 | |
| ViaSure H. pylori real-time PCR detection kit (CerTest Biotec S.L., Zaragoza, Spain) | SAT | 85.7 | 100 | 100 | 84.6 | 92 | 321 |
a
PPV, positive predictive value; NPV, negative predictive value.