TABLE 2.
Results of targeted metagenomics sequencing (tMGS) of whole blood, plasma, and both combineda
| Subjects | Positive tMGS test |
||
|---|---|---|---|
| Whole blood n = 34 |
Plasma n = 34 |
Combined n = 35 |
|
| All infective endocarditis, n positive/n total (%) | 20/34 (59%) | 16/34 (47%) | 23/35 (66%) |
| Blood culture positive infective endocarditis, n positive/n total BCPE (%) | 17/28 (61%) | 13/29 (45%) | 18/29 (62%) |
| Blood culture positive on day tMGS collected, n positive/subgroup total (%) | 7/7 (100%) | 6/7 (86%) | 7/7 (100%) |
| Blood culture negative on day tMGS collected, n positive/subgroup total (%) | 10/21 (48%) | 7/22 (32%) | 11/22 (50%) |
| Blood culture negative infective endocarditis, n positive/n total BCNE (%) | 3/6 (50%) | 3/5 (60%) | 5/6 (83%) |
a
Positivity was determined according to the decision tree shown in Fig. 2. For one subject, there was no plasma and for another no whole blood available for testing. BCPE, Blood culture-positive endocarditis. BCNE, Blood culture-negative endocarditis.