TABLE 3.

Description of cases and targeted metagenomic sequencing (tMGS) results in blood culture negative infective endocarditis (IE) cases

Subject	Case description	tMGS-whole blood	tMGS-plasma	Confirmation by duplicate tMGS testing
2	Definite mechanical aortic valve IE with new vegetations two months after treatment for Cardiobacterium hominis IE	Kingella sp. (most closely related to Kingella denitrificans)	Negative	No
12	Definite mechanical aortic valve IE	Bartonella sp.	Bartonella sp.	Yes
13	Possible native tricuspid valve IE in person who injected drugs	Negative	Cutibacterium acnes	Yes
17	Possible bioprosthetic aortic valve IE three months after previously treated Staphylococcus aureus IE	Negative	Corynebacterium sp.	Yes
26	Definite mechanical aortic and mitral valve IE plus native tricuspid valve IE after previously treated S. aureus aortic IE	Negative	Negative	Not applicable
35	Definite native tricuspid valve IE two months after treated Streptococcus dysgalactiae IE in the presence of a pacemaker	Streptococcus salivarius /thermophilus	Not tested	Yes