TABLE 2.
Plasmids used in this study
| Plasmid | Description [reference] |
|---|---|
| pALS10 | pBR322 derivative bearing the full-length relA; Apr [29, 32] |
| pALS13 | Like pALS10 but bearing the first 455 codons of relA [29, 32] |
| pALS14 | Like pALS10 but bearing the first 331 codons of relA [29, 32] |
| pALS10-G251E | Like pALS10 but containing the G251E mutation in relA |
| pALS13-G251E | Like pALS13 but containing the G251E mutation in relA |
| pALS10-H354Y | Like pALS10 but containing the H354Y mutation in relA |
| pNT8 | pKK-233-2 derivative encoding the truncated CTD of RelA (aa 497–744) |
| pYG4 | pKK-233-2 derivative encoding the truncated CTD of RelA (aa 564–675) |
| pYG4-C612G | Like pYG4 but containing the C612G mutation |
| pYG4-D637R | Like pYG4 but containing the D637R mutation |
| pYG4-C638F | Like pYG4 but containing the C638F mutation |
| pALS10-C612G | Like pALS10 but containing the C612G mutation |
| pALS10-D637R | Like pALS10 but containing the D637R mutation |
| pALS10-C638F | Like pALS10 but containing the C638F mutation |
| pT25 | Expression vector encoding the T25 fragment of CyaA in frame with a multi-cloning site; Cmr [14] |
| pT18 | Expression vector encoding the T18 fragment of CyaA in frame with a multicloning site; Apr [14] |
| PT25-RelA | PT25 derivative bearing the full-length relA (codons 1–744) |
| PT18-RelA | pT18 derivative bearing the full-length relA (codons 1–744) |
| pT25-CTD | pT25 derivative encoding the CTD of RelA (aa 564–744) |
| pT18-CTD | pT18 derivative encoding the CTD of RelA (aa 564–744) |
| pT18-NTD | pT18 derivative encoding the NTD of RelA (aa 1–456) |
| pT18-CTD-C612G | Like pT18-CTD but containing the C612G mutation |
| pT18-CTD-D637R | Like pT18-CTD but containing the D637R mutation |
| pT18-CTD-C638F | Like pT18-CTD but containing the C638F mutation |
| pT25-CTD long | pT25 derivative encoding the extended CTD of RelA (aa 564–744) |
| pT18-CTD long | pT18 derivative encoding the extended CTD of RelA (aa 564–744) |