TABLE 3.

Primers used in this study

Primer	Sequence (5′ → 3′)
G251E-5′	GTTAAAGCGGAAGTGTATGAGCGTCCGAAACACATCa
G251E-3′	GATGTGTTTCGGACGCTCATACACTTCCGCTTTAAC
EspI-5′	GAGTGCTTAGCCGAAACCTGGb
NsiI-3′	TTCATGCATCTGTTTGGTGCG
H354Y-5′	GTTGGGTGTTGCTGCGTACTGGAAATATAAAG
H354Y-3′	CTTTATATTTCCAGTACGCAGCAACACCCAAC
BamHI-3′	CTCTAGAGGATCCCCTATGTAG
Yg4 (PstI)	TGCACTGCAGTTAATAAGCCGAGTGCCG
B3(HindIII)	GCGACGAAGCTTCTAACTCCCGTGCAACCG ACGCGC
C612G-5′	GCACCACATCGCGCGCGGCTGCCAGCCGATTC
C612G-3′	GAATCGGCTGGCAGCCGCGCGCGATGTGGTGC
D637R-5′	GTATTTCAGTACACCGCGCCCGTTGCGAACAACTGGCGG
D637R-3′	CCGCCAGTTGTTCGCAACGGGCGCGGTGTACTGAAATAC
C638F-5′	CAGTACACCGCGCCGATTTCGAACAACTGGCGGAAC
C638F-3′	CAGTTCCGCCAGTTGTTCGAAATCGGCGCGGTGTAC
NsiI-5′	CAAATCCGCACCAAACAGATGCATGAAG
N-up	CTGCAGGTACCTATGGTTGCGGTAAGAAGT
N-down	TGAGGGTACCTGCAGCTGGTAGGTGAACGG
C-up	CTGCAGGTACCTTTTAATAAGCCGAGTGCCG
C-down	TGACGGTACCCGACTCCCGTGCAACCGACGC
C-long-up	CTGCAGGTACCTTACCAGCTGCAGATGGGCG
N-long-down	TGAGGGTACCTGATTAAATTGCGATTGCAGG

^aMutated bases are indicated in bold type. 

^bRestriction sites are underlined.