TABLE 4.
CyaA complementation of the bacterial two-hybrid system fusion plasmids
| Plasmids | Phenotype on MacConkey-maltosea at:
|
β-galactosidase activity (U/ml)b | ||
|---|---|---|---|---|
| 36 h | 48 h | 72 h | ||
| None | − | − | − | 101 |
| pT25-zip + pT18-zip | +++ | 3,961 | ||
| pT25-RelA + pT18-RelA | ++ | +++ | 3,310 | |
| pT25-CTD + pT18-RelA | ++ | +++ | 2,951 | |
| PT25-NTD + pT18-RelA | − | − | + | 1,371 |
| pT25-CTD + pT18-CTD | ++ | +++ | 3,606 | |
| pT25-CTD + pT18-CTD-C612G | − | − | + | 2,227 |
| pT25-CTD + pT18-CTD-D637R | − | ++ | +++ | 2,662 |
| pT25-CTD + pT18-CTD-C638F | − | − | + | 1,725 |
| pT25-CTD + pT18-NTD | − | − | − | 54 |
| pT25-CTD long + pT18-NTD | − | − | − | 111 |
| pT25-NTD + pT18-CTD long | − | − | − | 109 |
a
The intensity of the color of the colony: −, white; +, light red; ++, red; +++, dark red.
b
The results represent the average values obtained from at least four independent experiments.