Fig. 2. Specificity test of anti-PD-1 nanoMIP. The amounts of different peptides (A) and different proteins (B) captured by anti-PD-1 nanoMIP and nanoNIP; (C) binding isotherms of anti-PD-1 nanoMIP to PD-1 epitope peptide (Hill fitting: log[θ/(1−θ)] = logKd − nlog[free concentration]); (D) The BLI binding curves of anti-PD-1 nanoMIP towards recombinant PD-1 protein. Error bars represent three parallel experiments.
