Fig. 3. Anti-PD-1 nanoMIP blocked PD-1/PD-L1 interaction in protein and cellular level. (A) Schematic illustration of ELISA analysis of anti-PD-1 nanoMIP blocking the interaction of PD-1 and PD-L1; (B) ELISA result of blocking the PD-1 interaction with PD-L1; (C) Schematic illustration of flow cytometry analysis of anti-PD-1 nanoMIP blocking the PD-1/PD-L1 interaction on cell surface; (D) Flow cytometry of 786-O cells stained with PE-labelled PD-L1 with different pretreatments. Error bars represent three parallel experiments.