T _m values of FKBP12 with and without compounds^a.

Compounds	T m (°C)	ΔTmc (°C)
None	65.57 ± 0.04b
#4–1	68.35 ± 0.09	+2.78 ± 0.13
#8–1	68.55 ± 0.07	+2.98 ± 0.11
#8–2	68.27 ± 0.08	+2.70 ± 0.22
FK506d	78.81 ± 0.06	+13.20 ± 0.10

^a T _m values evaluated by protein thermal shift (PTS) assay with the standard error of the mean for the T_m values calculated by quintuple experiments.

^b T _m value of FKBP12 in the absence of any compounds.

^cDifference of the average T_m values of FKBP12 in the presence and absence of the analyte compounds (ΔT_m = T_m^b − T_m).

^dPositive control.