Table 1.
Genotype | Explants | Regeneration (%) 1 | dsRed + buds (%) 2 | Rooted lines | TE 3 |
---|---|---|---|---|---|
M2 | 300 | 288 (96) | 22 (7.3) | 10 | 3.33 |
M9 | 300 | 250 (83.4) | 29 (9.7) | 6 | 2.00 |
C‐46 | 300 | 215 (71.7) | 32 (10.7) | 2 | 0.67 |
M5 | 300 | 262 (87.4) | 45 (15) | 4 | 1.33 |
Regeneration: number of explants with, at least, one regenerating bud. Between brackets the regeneration rate: regeneration/total number of infected explants × 100.
We used the vector pMOG800, which expresses dsRed after the 35S promoter. Consigned are buds with dsRed expression/total number explants × 100.
Transformation efficiency (TE): number of rooted lines/total number explants × 100.