Table 2.

Method Qualification Data for Total Analytes in Male SD Rat Plasma

Compound	Concentration Range (ng/mL)	LODa (ng/mL)	LOQb (ng/mL)	Standard c		QCd
				Intra-day Accuracy (RE, %)e	Intra-day Precision (RSD, %)f	Intra-day Accuracy (RE, %)e	Intra-day Precision (RSD, %)f
P-BZT	10–1000	1.95	10.1	−14.3 to 5.9	1.4 to 18.0	−5.1 to −1.5	8.8 to 15.1
Octrizole	5–100	1.53	5.0	−7.5 to 11.4	0.8 to 9.1	−45.5 to 2.4	8.6 to 27.8
Drometrizole	1–100	0.14	1.0	−8.8 to 4.6	0.4 to 5.0	−25.2 to −3.6	15.8 to 17.3
tBuPrOcEst-BZT	1–100	0.12	1.0g	−7.1 to 4.5 g	0.0 to 7.0	−12.2 to 0.7g, −27.3 to −20.5h	6.7 to 10.2g 2.9 to 6.0h
Bumetrizole	1–100	NA	1.0	−8.1 to 17.8	5.5 to 7.3	−17.0 to 0.5	23.1 to 28.1
DiMeEtPh-BZT	1–100	0.22	1.0	−11.5 to 14.0	2.7 to 17.1	3.0 to 4.4	9.3 to 25.7
ditBuCl-BZT	1–100	0.04	1.0	−3.4 to 3.0	1.0 to 8.2	8.5 to 3.2	5.6 to 10.0
DitPe-BZT	5–500	NA	5.0	−18.2 to 17.2	5.0 to 12.9	8.0 to 12.7	4.2 to 8.4
tBu-BZT	1–100	NA	1.0	−13.2 to 11.9	1.6 to 12.4	2.4 to 4.0	8.4 to 23.9

^aLOD= Limit of Detection; 3×Standard Deviation for 6 lower limit of quantitation (LOQ) replicates

^bLOQ=lower limit if quantitation; is the lowest concentration of the calibration curve

^cEstimated based on plasma calibration standards, n = 2

^dEstimated based on low and high-level Quality Control (QC) samples, n = 4; 20 and 800 ng/mL for P-BZT; 9 and 75 ng/mL for Octrizole, 2 and 50 ng/mL for drometrizole, bumetrizole, DiMeEtPh-BZT, and ditBu-BZT; 10 and 250 ng/mL for DitPe-BZT; and 1.5 and 80 ng/mL for total tBuPrOcEst-BZT (as tBuPrOH-BZT))

^eRE %= percent relative error

^f%RSD = percent relative deviation

^gWith respect to tBuPrOH-BZT

^hWith respect to tBuPrOcEst-BZT