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. 2001 Jan;183(2):597–603. doi: 10.1128/JB.183.2.597-603.2001

FIG. 1.

FIG. 1

Immunoblotting analysis of culture supernatants (A) and cell lysates (B) from P. aeruginosa PABST7.1 expressing wild-type or cysteine variant lipases. Aliquots of culture supernatants of P. aeruginosa PABST7.1 expressing wild-type (bearing plasmid pUCPL6A) or cysteine-to-serine variants of lipase were subjected to SDS-PAGE and immunoblotting. Cell lysates were prepared by boiling aliquots of the cultures corresponding to an OD580 of 0.15 in sample buffer. Samples were subjected to SDS-PAGE and immunoblotting. A polyclonal antiserum raised against a peptide consisting of amino acids 48 to 60 in mature P. aeruginosa lipase was used to detect lipase. P. aeruginosa PABST7.1(pUCPKS) and purified lipase served as negative and positive controls, respectively.