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. 2022 Sep 21;20(9):e3001764. doi: 10.1371/journal.pbio.3001764

Fig 2. MYORG is a dimeric α-galactosidase that shows distinct substrate specificity.

Fig 2

(a) SEC-MALLS traces of glycosylated and EndoH-treated MYORG. (b) Fluorescent activity assay of MYORG against 4MU-α-linked substrates. Data is mean from 3 technical replicates ± standard deviations. (c) Example isothermal titration calorimetry trace of DGJ binding to MYORG. (ci) Raw baseline subtracted injection profile of the ITC experiment. (cii) Titration curve with points in blue and fitted line in black. (d) Activity screening of MYORG against disaccharides. Experiment repeated twice with 3 technical repeats in each replicate. (e) Michaelis–Menten kinetics for processing of Gal-α1-4-Glc by MYORG. Data from 3 technical repeats. All raw data underlying graphs can be found in S1 Data. BGBT, blood group B trisaccharide; DGJ, deoxygalactonojirimycin; MYORG, myogenesis-regulating glycosidase.