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. Author manuscript; available in PMC: 2023 May 9.
Published in final edited form as: Curr Biol. 2022 Mar 24;32(9):1924–1936.e6. doi: 10.1016/j.cub.2022.03.005

Figure 5. Different neuronal types carry distinct EV cargo; a single neuronal cilium may shed multiple cargoes.

Figure 5.

(A) MCM-3::mScarlet is present in cilia of PKD-2-expressing and IL2 neurons. Circles indicate MCM-3::Scarlet (magenta) and PKD-2::GFP EVs (B-C) TSP-6::mScarlet is present in amphid channel and AWA neurons of the head (C) and is released in ciliary EVs into the environment (D). Amphid channel is outline with white dashed line. Images are shown as projected 3D objects with the use of actual pixel values in a transparent volume. Images show 3D renderings: on merged images green and magenta colors represent different reporters, on images showing individual channels colors code for Z-depth - 5.0 μm for (A) and 6.0 μm for (C). Black and white maximum intensity projections for (A-C) and other data related to MCM-3::mScarlet and TSP-6::mScarlet expression patterns are included in Figure S5.