Figure 3.

The DL workflow of Aβ₄₂ and pS396-Tau deposits prediction. (a) (1) The process of retina cross-sections preparation: donor eye fixation, neurosensory retina isolation to flatmounts, creating four retinal quadrants (S: superior, T: temporal, I: inferior, N: nasal), and sectioning of ST and IT strips. (2) The retina cross-sections are imaged by our HSI microscope before immunostaining. Raw data are normalized and go through PCA process to convert to RGB images. (3) and (4) are two different staining techniques used for comparison of the HSI analysis. (b) HSI images are registered with fluorescence and DAB staining images. Patches of size 256 × 256 pixels are cropped from the HSI and staining images. Corresponding images form training pairs for the GAN. (c) We input the ROI patches to the trained model and get the inference patches for Aβ₄₂ and pS396-Tau with different immune contrasts.