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. 2022 Sep 21;8(38):eabn6545. doi: 10.1126/sciadv.abn6545

Fig. 1. TMEM176B is a protective host factor in β-coronavirus infection by controlling inflammasome activation.

Fig. 1.

(A) Heatmaps of indicated transcripts. Analysis of published data (36). (B) Correlation study ofTMEM176B/glyceraldehyde-3-phosphate dehydrogenase (GAPDH) ratio in CD14+ cells with plasmatic active caspase-1. AU, arbitrary units. (C) E protein triggers IL-1β secretion by THP-1 monocytes. One experiment representative of three is shown. **P < 0.01, Student’s t test. (D and E) IL-1β secretion and active caspase-1 triggered by E protein were inhibited by TMEM176B in THP-1 monocytes. One experiment representative of three is shown in (D), and a pool of two experiments is shown in (E). *P < 0.05, Student’s t test. (F) Survival of MHV-A59–infected mice. *P < 0.05, log-rank (Mantel-Cox) test. (G) Viral load in the liver at 5 days post-infection (dpi). *P < 0.05, Student’s t test. (H) Serum plasma glutamic-pyruvic transaminase (GPT) transaminase activity at 5 dpi. *P < 0.05, Student’s t test. (I) Western blot study in the liver at 5 dpi. (J) Semiquantification of Western blot bands. *P < 0.05; **P < 0.01; ***P < 0.001, two-way analysis of variance (ANOVA) test. (K and L) Percentage of FLICA-1+ cells within CD11c+ MHC II+ CD11b− and CD11b+ cells. *P < 0.05; ****P < 0.0001, Student’s t test. (M and N) Viral load in the liver at 5 dpi. *P < 0.05; **P < 0.01, one-way ANOVA test. (O) Survival in infected mice. *P < 0.05; ***P < 0.001; ****P < 0.0001, log-rank (Mantel-Cox) test.