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. 2022 Sep 21;8(38):eabo0987. doi: 10.1126/sciadv.abo0987

Fig. 2. PKM2 deletion attenuates BLM-induced pulmonary fibrosis.

Fig. 2.

(A) Schema illustrating generation of PKM2-KO mice. PKM2-KO and control mice were subjected to BLM treatment and euthanized at the indicated time points. (B) H&E staining of lung sections from control and PKM2-KO mice at various time points after BLM induction. Scale bars, 2 mm (top panel) and 100 μm (bottom panel). (C) Quantification of the severity of fibrosis. The fibrotic area is presented as percentage (n = 10 per group). (D) Masson’s staining of collagen on lung sections from control and PKM2-KO mice at various time points after BLM induction. Scale bars, 2 mm (top panel) and 100 μm (bottom panel). (E) Hydroxyproline content in lung tissues in control and PKM2-KO mice at various time points after BLM induction (n = 10 per group). (F to G) Representative results (n = 5 of Western blot with n = 10 mice per group) of α-SMA, Col1, and Fn (F); p-Smad3, Smad3, p-Akt, Akt, p-Erk, Erk, p-JNK, JNK, p-P38, and P38 (G) expression in lung tissues from control and PKM2-KO mice at various time points after BLM induction. Data are represented as the means ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001, Student’s t test.